Wednesday, December 1, 2010

Materials and methods


This project focuses on the development of the ECG component that is capable of capturing the ECG signal from human body. In ECG measurement portion, sensing bio electric potential is necessary. For this electrodes were used. Figure 6 shows the 3M Red Dot Repositionable Electrodes that were used in this project.


 The 3M Red Dot Repositionable Electrode is comfortable and breathable with a soft cloth backing, therefore they are conformable to skin contours. Red Dot Repositionable Electrodes reduce artifact, and may offer time and cost savings. The entire surface of the electrode is conductive, resulting in a low impedance electrode. For adhesive we used ECG jelly (STERIMED) which is non irritating to skin, has neutral pH and high conductivity.

The ECG signal from the body was amplified using instrumentation amplifier (AD620). AD620 is the bio-potential amplifier used in the system. It has gain range of 1 to 1000, low power, common Mode Rejection Ratio (CMMR) greater than 100dB. It requires only a single external gain setting resistor Rg. The signal after amplification was filtered through Low Pass Filter (LPF), followed by High Pass Filter (HPF) and lastly Notch filter. For the design of these filter, various combination of resistor and capacitor were used using operational amplifier (TL074CN). It is a quad J-fet operational amplifier with low power consumption and wide CMMR. The Filtered ECG signal was then viewed in computer using Sound Oscilloscope (C. Zeitnitz). The stress to the heart is given through motorized Treadmill Machine (WNQ) as shown in Figure 7.
  

Electrocardiogram Artifacts


ECG artifacts are common and include tracings recorded from external ECG machines, bedside and central station monitors, treadmill ECG machines, and ambulatory rhythm monitors such as Holter recordings. ECG artifacts have variable causes and include body motion of or near the skin electrodes, such as scratching over an electrode, circuitry problems, such as inadequate electrode connections or disconnections; and deliberate patient manipulation of the body surface electrodes. Signal processing of exercise ECG is an issue and artifacts are a recurring problem. It is difficult to discriminate the ECG curves from artifacts, especially in exercise ECGs and particularly in the high exercise phase.
Motion artifact, the most prevalent and difficult type of noise to filter in exercise ECG, corrupts the intelligibility of the desired signal thus reducing the reliability of stress test. The patient movement is the catalyst for motion artifact, measuring body movement by means of an accelerometer and using the sensor measurement in an adaptive filtering system to remove or at least minimize the noise reduces the amount of motion artifact in stress ECG.

Timeline development of ECG


Alexander Muirhead attached wired to a feverish patient’s wrist to obtain a record of the patient’s heartbeat while studying for his doctor of science (in electricity) in 1872 by Hospital. This activity was directly recorded and visualized using a Lippnann capillary electrometer by the British physiologist John Burdon Sanderson .

The first to systematically approach the heart from an electrical point-of-view was Augustus Waller, working in St. Mary’s Hospital in Paddington, London. His electrocardiograph machine consisted of Lippmann capillary electrometer fixed to projector. The trace from the heart beat was projected onto a photographic plate which was itself to a toy train. This allowed a heartbeat to be recorded in real time. In 1911 he still saw little clinical application for his work.

British physiologists William Bayliss and Edward Starling of University College London in 1892 improved the capillary electromotor. They connect the terminals to the right hand and to the skin over the apex beat and show a “triphasic variation accompanying (or rather preceding) each beat of the heart”. These deflections are later called P, QRS, and T. They also demonstrated delay of about 0.13 seconds between trial stimulation and ventricular depolarization (later called PR interval) .

The breakthrough came when Willem Einthoven, working in Leiden, The Netherlands, used the string galvanometer which he invented in 1902, which was much more sensitive than capillary electrometer that Waller used. Einthoven assigned the letters P, Q, R, S and T to the various deflections, and described the electrocardiographic features of a number of cardiovascular disorders. In 1924, he was awarded the Nobel Prize in Medicine for his discovery.

Einthoven discussed commercial production of a string galvanometer with Max Edelmann of Munich and Horace Darwin of Cambridge Scientific Instrument Company of London in 1903. He also started transmitting electrocardiograms from Hospital to his laboratory 1.5 km away via telephone cables. On March 22nd 1905 the first ‘telecardigram’ was recorded from a healthy and vigorous man and the tall R waves are attributed to his cycling from laboratory to hospital for recording. ECG sensor design is traditionally completed in the field of biomedical Engineering. Investigation of Biomedical Engineering represents a good starting point for a person embarking on research of ECG.

Types of ECG


a. Resting ECG:
The patient lies down for few minutes while a record is made. In this type of ECG, disks are attached to the patient’s arms and legs as well as to the chest.
b. Exercise ECG (stress test):
The patient exercises either on treadmill machine or bicycle while connected to the ECG machine. This test tells whether exercise causes arrhythmias or makes them worse or whether there is evidence of inadequate blood flow to the heart muscle (ischemia).

c. 24-Hour ECG (Holter) monitoring:
This is a sophisticated type of ECG. The patient goes about his or her usual daily activities while wearing a small, portable tape recorder that connects to the disks on the patient’s chest. Overtime this test shows changes in rhythm (or ischemia) that may not be detected during a resting or exercise ECG . The most commonly used ECG is the Resting ECG. For less frequent or hard to predict arrhythmias, loop recorders are used. These are worn by the patient for weeks to even as long as month. When the patient feels an uncomfortable heart rhythm start up he/she presses a button which records his/ her ECG for a fixed duration.
ECG supplies are used when monitoring a patient for a resting or diagnostic ECG analysis, stress testing and exercise monitoring, ambulatory monitoring and other imaging procedures. Also, gels, wipe pads and paper are used during an ECG measurement.

The word lead has two meanings in electrocardiography: it refers to either the wire that connects an electrode to the electrocardiograph, or (more commonly) to a combination of electrodes that form an imaginary line in the body along which the electrical signals are measured.
An electrocardiogram is obtained by measuring electrical potential between various points of the body using a biomedical instrumentation amplifier. A lead records the electrical signals of the heart from a particular combination of recording electrodes which are placed at specific points on the patient’s body. When a depolarization wave front (or mean electrical vector) moves towards a positive electrode, it creates a positive deflection on the ECG in the corresponding lead.
When a depolarization wave front moves away from a positive electrode, it creates a negative deflection on the ECG in the corresponding lead. When a depolarization wavefront moves perpendicular to a positive electrode, it creates an equiphasic (or isoelectric) complex on the ECG [24]. In standard ECG recording there are five electrodes connected to the patient:
1. Right arm, RA
2. Left arm, LA
3. Left leg, LL
4. Right leg, RL
5. Chest, C
Depending how the electrodes pairs are connected to the ECG sensor different waveforms and amplitudes can be obtained. Each pair contains unique information of the heart activity that cannot be obtained from another pair of leads.
The different leads are divided into groups depending how they are connected to the ECG amplifier. There are two types of leads-Unipolar and Bipolar. The former have an indifferent electrode at the center of the Einthoven’s triangle (which can be likened to the ‘neutral’ of a wall socket) at zero potential. The direction of these leads is from the center .

The bipolar type, in contrast, has both electrodes at some potential, with  the direction of the corresponding lead being from the electrode at lower potential to the one at higher potential, e.g. in limb lead I, the direction is from left to right. These include the limb leads-I, II and III.
Lead I, II and III are the so-called limb leads because at one time, the subjects of electrocardiography had to literally place their arms and legs in buckets of salt water in order to obtain signals for Einthoven’s triangle. Eventually, electrodes were invented that could be placed directly on the patient’s skin. Even though the buckets of salts water are no longer necessary, the electrodes are still placed on the patient’s arms and legs to approximate the signals obtained with the buckets of salt water. They remain the first three lead of modern 12 lead ECG.
Lead I is a dipole with the negative electrode on the right arm and the positive electrode on the left arm.
Lead II is a dipole with the negative electrode on the right arm and the positive electrode on the left leg.
Lead III is a dipole with the negative electrode on the left arm and the positive electrode on the left leg.



Leads aVR, aVL, and aVF are augmented limb leads. They are derived from the same three electrodes as leads I, II and III. However, they view the heart from different angles ( or vectors)  because the negative electrode for these leads is a modification of Wilson’s central terminal, which is derived by adding leads I, II ,III together and plugging them into the negative terminal of the ECG  machine. This zeroes out the negative electrode and allows the positive electrode to become the “exploring electrode” or a unipolar lead. This is possible because Einthoven’s Law states that 
I+(-II)+III=0.....................equation (i)
I+III=II..............................equation (ii)
Where, I, II, III are leads.
It is written this way instead of I+II+III=0 because Einthoven reversed the polarity of lead II in Einthoven’s triangle, possibly because he liked to view upright QRS complexes. Wilson’s central terminal paced the way for the development of the augmented limb leads aVF, aVL, aVF and the precordial leads V1, V2, V3, V4, V5 and V6.
Lead aVR or “augmented vector right” has the positive electrode on the right arm. The negative electrode is a combination of the arm electrode and the left leg electrode, which “augments” the signal strength of the positive electrode on the right arm.
Lead aVL or “augmented vector left has the positive electrode on the left arm. The negative electrode is a combination of the right arm electrode and the left leg electrode, which “augments” the signal strength of the positive electrode on the left arm. Lead aVF or “augmented vector foot” has the positive electrode on the left leg. The negative electrode is a combination of the right arm electrode and the left arm electrode, which “augments” he signal of the positive electrode on the left leg. 
The augmented limb lead a VR, aVL, and aVF are amplified in this way because the signal is too be useful when the negative electrode is Wilson’s central terminal. Together with lead I, II, III augmented limb leads a VR, aVL, and a VF form the basis of the hexaxial reference system, which is used to calculate the heart’s electrical axis in the frontal plane .



The precordial leads V1, V2, V3, V4, V5 and V6 are placed directly on the chest. Because of their close proximity to the heart, they do not require augmentation. Wilson’s central terminal is used for the negative electrode, and these leads are considered to be unipolar. The precordial leads view the heart’s electrical activity in the so-called horizontal plane. The heart’s electrical axis in the horizontal plane is referred to as the Z-axis.
Leads V1, V2 and V3 are referred to as the right precordial leads and V4, V5 and V6 are referred to the left precordial leads.
The QRT complex should be negative in lead V1 and positive in lead V6. The QRT complex should show a gradual transition from negative to positive between leads V2 and V4. The equiphasic lead is referred to as the transition lead. When the transition occurs earlier than lead V3, it is referred to as an early transition. When it occurs later than lead V3, it is referred to as late transition. There should also be a gradual increase in the amplitude of the R wave between leads V1 and V4. This is known as T wave progression. Poor R wave progression is a non-specific finding. It can be caused by conduction abnormalities, myocardial infarction, cardiomyopathy, and other pathological conditions .

                                                                                            

Lead V1 is placed in the fourth intercostal space to the right of the sternum.
Lead V2 is placed n the fourth intercostal space to the left of the sternum.
Lead V3 is placed directly between leads V2 and V4.
Lead V4 is placed in the fifth intercostal space in the midclavicular line (even if the apex beat is displaced).
Lead V5 is placed horizontally with V4 in the anterior axillary line.
Lead V6 is placed horizontally with V4 and V5 in the mid axillary line.

An additional electrode is present in modern three-lead and twelve-lead ECGs. This is the ground lead and is placed on the right leg by convention, although in theory it can be placed anywhere on the body. With a three-lead ECG, when one dipole is viewed, the remaining lead becomes the ground lead by default.

ECG Background


An electrocardiogram (ECG) is noninvasive transthoracic graph produced by an electrocardiograph, which record the electrical activity of the heart over time. Its name is made of different parts: electro, because it is related to electrical activity, cardio, Greek for heart, gram a Greek roots meaning “to write” .

Electrical impulse in the heart originates in the sinoatrial node and travel through the intrinsic conducting system to the heart muscle. The impulses stimulate the myocardial muscle fibers to contract and thus induce systole. The electrical waves can be measured at electrodes placed at specific points on the skin. Electrodes on different sides of the heart measure the activity of different parts of the heart muscle.

William Einthoven developed the first electrocardiogram in 1903 using a crude galvanometer. Technology has advanced ECG measurement, but the principle remains the same. The electrocardiogram is the wave representation of the potential difference caused by heart activity. A grasp of the electrocardiogram has to be gained for two reasons:
1.      An understanding of the wave forms the basis for the design of the electronic circuit to measure it; and
2.      An understanding allows the concept of what and ECG is, and how its deviation enables analysis of health.

Protocols used in TMT


a. Bruce Protocol :
Before the development of the Bruce Protocol there was no safe, standardized protocol that could be used to monitor cardiac function in exercising patients. Most physicians relied upon patients complaints about exertion, and examined them only at rest. To address these problems, Dr. Robert A. Bruce and Dr. Paul Yu began work on developing a treadmill exercise test. The test made extensive use of relatively new technological developments in electrocardiographs and motorized treadmills.

A  Bruce exercise test involved walking on a treadmill while the heart was monitored by an electrocardiograph with various electrodes attached to the body. Ventilation volumes and respiratory gas exchanges were also monitored, before, during and after exercise. Because the treadmill speed and inclination could be adjusted, this physical activity was tolerated by most patients. Initial experiments involved a single-stage test, in which subjects walked for 10 minutes on the treadmill at a fixed workload. Bruce analyzed minute-by-minute changes in respiratory and circulatory function of normal adults and patients with heart or lung ailments. Later he developed the multistage test, consisting of several stages of progressively greater workloads. It was this multistage test, which later became known as the Bruce protocol. In the initial experiment, Bruce reported that the test could detect signs of such conditions as angina pectoris, a previous heart attack, or a ventricular aneurysm. Bruce also demonstrated that exercise testing was useful in screening apparently healthy people for early signs of coronary artery disease .

Typically during a Bruce Protocol, Heart Rate (HR) is taken every minute and BP is taken at the end of each stage (every three minutes). However institutions often vary this procedure slightly.
The Bruce Treadmill Test Protocol
Level
Time (mins)
Speed (km/hr)
Gradient (%)
1
0
2.74
10
2
3
4.02
12
3
6
5.47
14
4
9
6.76
16
4
12
8.05
18
5
15
8.85
20
6
18
9.65
22
So, from the chart above, we see that the test starts at 2.7 km/hr at a gradient or incline of 10%. At minute 3 the speed is increased to 4.02km/hr and the gradient increased to 12%. This is a maximal test which means that the individual must continue until fatigued .Needless to say in a clinical setting; other parameters (such as blood pressure and ECG readings etc.) are used to determine the end of the test . Today, the Bruce Protocol is also one common method for estimating VO2 maximum in athletes. VO2 maximum, or maximal oxygen uptake, is one factor that can determine an athlete’s capacity to perform sustained exercise and is linked to aerobic endurance. VO2 maximum refers to the maximum amount of oxygen that an individual can utilize during intense or maximal exercise. It is measured as “ milliliters of oxygen used in one minute per kilogram of body weight”(ml/kg/min).The Bruce treadmill test is an indirect test that estimates VO2 maximum using a formula rather than using direct measurements that require the collection and measurement of the volume and oxygen concentration of inhaled and exhaled air. This determines how much oxygen the athlete is using. The length of time on the treadmill is the test score and can be used to estimate the VO2 maximum value . The Bruce Protocol Formula for estimating VO2 maximum:
·                     For Men, VO2 maximum=14.8-(1.379*T)-(0.012*T)
·                     For Women, VO2 maximum=4.38*T-3.9
Where, T=Total time on the treadmill measured as a fraction of a minute (i.e: A test time of 9 minutes would be written as T=9.5).
Men
Age
Very Poor
Poor
Fair
Good
Excellent
Superior
13-19
<35.0
35.0-38.3
38.4-45.1
45.2-50.9
51.0-55.9
>55.9
20-29
<33.0
33.0-36.4
36.5-42.4
42.5-46.4
46.5-52.4
>52.4
30-39
<31.5
31.5-35.4
35.5-40.9
41.0-44.9
45.0-49.4
>49.4
40-49
<30.2
30.2-33.5
33.6-38.9
39.0-43.7
43.8-48.0
>48.0
50-59
<26.1
26.1-30.9
31.0-35.7
35.8-40.9
41.0-45.3
>45.3
60+
<20.5
20.5-26.0
26.1-32.2
32.3-36.4
36.5-44.2
>44.2


b.   Balke Protocol:
Balke Protocol has been used to monitor the development of the athlete’s general endurance (VO2 maximum). In this, athlete walks on a treadmill to exhaustion. At timed stages during the test the gradient of slope (%) of the treadmill is increased as follows .
 Active and sedentary men:
o        Treadmill speed set at 3.3 mph
o        Start –Gradient is 0%
o        After 1 minute-Gradient set at 12%
o        After 2 minutes and each minute thereafter the gradient is increased by 1 %

Active and sedentary women:
o        Treadmill speed set at 3.0 mpg
o        Start-Gradient is 0%
o        After 3 minutes and every 3 minutes thereafter the gradient is increased by 2.5%

The assistant starts the stop watch at the start of the test and stops it when the athlete is unable to continue –this ideally should be between 9 and 15 minutes. From the total time an estimate of the athlete‘s VO2 maximum can be calculated as follows:
VO2 maximum=1.44*T+14.99(for active and sedentary men)
VO2 maximum=1.38*T+5.22(for Active and sedentary women)
Where, “T” is the total time of the test expressed in minutes and fractions of a minute.